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Nucleic Acid Purification & Analysis> | … Reagents. Agarose (regular and low-melting). Agarose loading gel mixture with Ficoll (GenScript D0084). ATP (10 mm). BBW buffer (1×). Biotinylated internal adaptors T30B and T30 (Applied Biosystems): T30B sequence, 5′-phos-CGTACA/iBiodT/CCGCCTTGGCCGT … | Get A Quote |
Mapping open chromatin regions has emerged as a widely used tool for identifying active regulatory elements in eukaryotes. However, existing approaches, limited by reliance on DNA fragmentation and short-read sequencing, cannot provide information about large-scale chromatin states or reveal coordination between the states of distal regulatory elements. We have developed a method for profiling the accessibility of individual chromatin fibers, a single-molecule long-read accessible chromatin mapping sequencing assay (SMAC-seq), enabling the simultaneous, high-resolution, single-molecule assessment of chromatin states at multikilobase length scales. Our strategy is based on combining the preferential methylation ... More